Column measurements show that, despite the higher static capacity of Capto Core 400 for BSA, the dynamic binding capacity is greater for Capto Core 700 as a result of its faster kinetics. Adsorbed Tg further hinders diffusion of BSA in both resins. These values decrease dramatically for Tg to 0.022 × 10 -7 and 0.088 × 10 -7 cm 2/s and to 0.13 × 10 -7 and 0.59 × 10 -7 cm 2/s for Capto Core 400 and 700, respectively. The core of each bead is functionalized with hydrophobic and positively charged ligands. The inactive shell aids in preventing large molecules (cut off Mr 700 000) from entering the core via the pores of the shell. The inactive shell aids in preventing large molecules (cut off Mr 700 000) from entering the core via the pores of the shell. CaptoCore 700 comprises a ligand-activated core and inactive shell.
For BSA, core and shell effective pore diffusivities are about 0.25 × 10 -7 and 0.6 × 10 -7 cm 2/s, respectively, for Capto Core 400, and about 1.6 × 10 -7 and 2.6 × 10 -7 cm 2/s, respectively, for Capto Core 700. Capto Core 700 comprises a ligand-activated core and inactive shell. Mass transfer in both resins is affected by diffusional resistances through the shell and within the adsorbing core. Prepacked HiTrap columns for easy screening and. However, for the much larger Tg, the attainable capacity is substantially larger for Capto Core 700. Cytiva - 29400461 - HiTrap Capto Core 700, 1 × 1 mL High capacity and productivity in flowthrough mode. Because of the smaller pores and higher surface area, the BSA binding capacity of Capto Core 400 is approximately double that of Capto Core 700. Product name Capto Core 700, 100 ml Catalogue Number Other means of identificationProduct type 17548102 Not available.Liquid. Although shell thicknesses are comparable (3.6 and 4.2 µm for Capto Core 400 and 700, respectively), the two resins differ substantially in pore size (pore radii of 19 and 50 nm, respectively). Zoonotic Coronaviruses Regeneration of Capto Core 700 resin through high throughput and laboratory scale studies and impact on production of a SARS-CoV-2. Both resins are agarose-based and contain an adsorbing core surrounded by an inert shell. Structural and functional characteristics of the two core-shell resins Capto™ Core 400 and 700, which are useful for the flow-through purification of bioparticles such as viruses, viral vectors, and vaccines, are compared using bovine serum albumin (BSA) and thyroglobulin (Tg) as models for small and large protein contaminants.
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